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harmonic generator thg  (Inrad Inc)

 
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    Inrad Inc harmonic generator thg
    Harmonic Generator Thg, supplied by Inrad Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/harmonic+generator+thg/pm41378695-108-18-30?v=Inrad+Inc
    Average 86 stars, based on 1 article reviews
    harmonic generator thg - by Bioz Stars, 2026-08
    86/100 stars

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    (A) 2PEF and <t>(B)</t> <t>3PEF</t> image stacks of fluorescently labeled blood vessels in the spinal cord of a live mouse. (C) 2PEF (left column), 3PEF (middle column), and <t>THG</t> images (right column) at selected depths into the mouse spinal cord. (D) Line profiles across selected capillaries, comparing 3PEF (magenta) and 2PEF (blue) image contrast at different depths. Lines in (C) indicate location of lineouts. (E) SBR for 2PEF and 3PEF imaging of the spinal cord vasculature. In the center region of the frame, the SBR was calculated as the average pixel intensity of the upper 1% to the lower 5%. The line show fits to exponential decays, with the characteristic attenuation length (CAL) of the fit indicated.
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    (A) 2PEF and <t>(B)</t> <t>3PEF</t> image stacks of fluorescently labeled blood vessels in the spinal cord of a live mouse. (C) 2PEF (left column), 3PEF (middle column), and <t>THG</t> images (right column) at selected depths into the mouse spinal cord. (D) Line profiles across selected capillaries, comparing 3PEF (magenta) and 2PEF (blue) image contrast at different depths. Lines in (C) indicate location of lineouts. (E) SBR for 2PEF and 3PEF imaging of the spinal cord vasculature. In the center region of the frame, the SBR was calculated as the average pixel intensity of the upper 1% to the lower 5%. The line show fits to exponential decays, with the characteristic attenuation length (CAL) of the fit indicated.
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    (A) 2PEF and <t>(B)</t> <t>3PEF</t> image stacks of fluorescently labeled blood vessels in the spinal cord of a live mouse. (C) 2PEF (left column), 3PEF (middle column), and <t>THG</t> images (right column) at selected depths into the mouse spinal cord. (D) Line profiles across selected capillaries, comparing 3PEF (magenta) and 2PEF (blue) image contrast at different depths. Lines in (C) indicate location of lineouts. (E) SBR for 2PEF and 3PEF imaging of the spinal cord vasculature. In the center region of the frame, the SBR was calculated as the average pixel intensity of the upper 1% to the lower 5%. The line show fits to exponential decays, with the characteristic attenuation length (CAL) of the fit indicated.
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    (A) 2PEF and <t>(B)</t> <t>3PEF</t> image stacks of fluorescently labeled blood vessels in the spinal cord of a live mouse. (C) 2PEF (left column), 3PEF (middle column), and <t>THG</t> images (right column) at selected depths into the mouse spinal cord. (D) Line profiles across selected capillaries, comparing 3PEF (magenta) and 2PEF (blue) image contrast at different depths. Lines in (C) indicate location of lineouts. (E) SBR for 2PEF and 3PEF imaging of the spinal cord vasculature. In the center region of the frame, the SBR was calculated as the average pixel intensity of the upper 1% to the lower 5%. The line show fits to exponential decays, with the characteristic attenuation length (CAL) of the fit indicated.
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    (A) 2PEF and <t>(B)</t> <t>3PEF</t> image stacks of fluorescently labeled blood vessels in the spinal cord of a live mouse. (C) 2PEF (left column), 3PEF (middle column), and <t>THG</t> images (right column) at selected depths into the mouse spinal cord. (D) Line profiles across selected capillaries, comparing 3PEF (magenta) and 2PEF (blue) image contrast at different depths. Lines in (C) indicate location of lineouts. (E) SBR for 2PEF and 3PEF imaging of the spinal cord vasculature. In the center region of the frame, the SBR was calculated as the average pixel intensity of the upper 1% to the lower 5%. The line show fits to exponential decays, with the characteristic attenuation length (CAL) of the fit indicated.
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    Image Search Results


    (A) 2PEF and (B) 3PEF image stacks of fluorescently labeled blood vessels in the spinal cord of a live mouse. (C) 2PEF (left column), 3PEF (middle column), and THG images (right column) at selected depths into the mouse spinal cord. (D) Line profiles across selected capillaries, comparing 3PEF (magenta) and 2PEF (blue) image contrast at different depths. Lines in (C) indicate location of lineouts. (E) SBR for 2PEF and 3PEF imaging of the spinal cord vasculature. In the center region of the frame, the SBR was calculated as the average pixel intensity of the upper 1% to the lower 5%. The line show fits to exponential decays, with the characteristic attenuation length (CAL) of the fit indicated.

    Journal: bioRxiv

    Article Title: Three-photon excited fluorescence microscopy enables imaging of blood flow, neural structure and inflammatory response deep into mouse spinal cord in vivo

    doi: 10.1101/2024.04.04.588110

    Figure Lengend Snippet: (A) 2PEF and (B) 3PEF image stacks of fluorescently labeled blood vessels in the spinal cord of a live mouse. (C) 2PEF (left column), 3PEF (middle column), and THG images (right column) at selected depths into the mouse spinal cord. (D) Line profiles across selected capillaries, comparing 3PEF (magenta) and 2PEF (blue) image contrast at different depths. Lines in (C) indicate location of lineouts. (E) SBR for 2PEF and 3PEF imaging of the spinal cord vasculature. In the center region of the frame, the SBR was calculated as the average pixel intensity of the upper 1% to the lower 5%. The line show fits to exponential decays, with the characteristic attenuation length (CAL) of the fit indicated.

    Article Snippet: We used a commercial optical parametric amplifier (OPA) to generate 1,320-nm wavelength pulses for 3PEF and third harmonic generation (THG) imaging (Opera-F, Coherent).

    Techniques: Labeling, Imaging

    (A) 2PEF (left column), 3PEF (middle column), and THG images (right column) at selected depths into the mouse spinal cord. Scale bar: 100 μm. (B) Line profiles across selected capillaries, comparing 3PEF (magenta) and 2PEF (blue) image contrast at different depths. Lines in (A) indicate location of lineouts. These data include the three depths shown in and and add three additional depths. Scale bar: 100 μm.

    Journal: bioRxiv

    Article Title: Three-photon excited fluorescence microscopy enables imaging of blood flow, neural structure and inflammatory response deep into mouse spinal cord in vivo

    doi: 10.1101/2024.04.04.588110

    Figure Lengend Snippet: (A) 2PEF (left column), 3PEF (middle column), and THG images (right column) at selected depths into the mouse spinal cord. Scale bar: 100 μm. (B) Line profiles across selected capillaries, comparing 3PEF (magenta) and 2PEF (blue) image contrast at different depths. Lines in (A) indicate location of lineouts. These data include the three depths shown in and and add three additional depths. Scale bar: 100 μm.

    Article Snippet: We used a commercial optical parametric amplifier (OPA) to generate 1,320-nm wavelength pulses for 3PEF and third harmonic generation (THG) imaging (Opera-F, Coherent).

    Techniques:

    (A) Experiment timeline and schematics for 3PEF imaging of neural dieback and inflammatory response following a photothrombotic venule occlusion. (B) 3D reconstruction of a z-stack taken from a Thy1-YFP x Cx3Cr1-GFP double transgenic mouse spinal cord; vessels were labeled with i.v. injection of Qtracker TM 655 red-emitting quantum dots. Arrow points to the occluded vessel segment. (C) A cessation of blood flow after a green laser-induced photothrombotic occlusion was followed by rapid axon dieback (yellow, seen via 3PEF) and myelin degeneration (grey, seen via THG) nearby the targeted vessel segment. (D) Time-series of representative images across different laminar depths showing progressive neural dieback, inflammatory response, and capillary disruption after a surface venule occlusion.

    Journal: bioRxiv

    Article Title: Three-photon excited fluorescence microscopy enables imaging of blood flow, neural structure and inflammatory response deep into mouse spinal cord in vivo

    doi: 10.1101/2024.04.04.588110

    Figure Lengend Snippet: (A) Experiment timeline and schematics for 3PEF imaging of neural dieback and inflammatory response following a photothrombotic venule occlusion. (B) 3D reconstruction of a z-stack taken from a Thy1-YFP x Cx3Cr1-GFP double transgenic mouse spinal cord; vessels were labeled with i.v. injection of Qtracker TM 655 red-emitting quantum dots. Arrow points to the occluded vessel segment. (C) A cessation of blood flow after a green laser-induced photothrombotic occlusion was followed by rapid axon dieback (yellow, seen via 3PEF) and myelin degeneration (grey, seen via THG) nearby the targeted vessel segment. (D) Time-series of representative images across different laminar depths showing progressive neural dieback, inflammatory response, and capillary disruption after a surface venule occlusion.

    Article Snippet: We used a commercial optical parametric amplifier (OPA) to generate 1,320-nm wavelength pulses for 3PEF and third harmonic generation (THG) imaging (Opera-F, Coherent).

    Techniques: Imaging, Transgenic Assay, Labeling, Injection, Disruption